FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_28.351000000608be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_28.351000000608be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19868
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC1110.5586873364203745No Hit
AGAGGACGAGCTGTCTCTTATACACAT980.49325548620897924No Hit
AGAGGACGAGATCTGTCTCTTCTACAC410.2063619891282464No Hit
AGAGGACGAGATGCTGTCTCTTATACA300.15099657741091202No Hit
AGAGGACGAGCTGTCTCTTATCCACAT290.14596335816388162No Hit
AGAGGACGAGATGGGGGCTGTCTCTTA220.11073082343466881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGA17250.021.561043
AGTGGAC403.9542792E-621.5610391
CGGACGA1200.021.5610393
AGCGGAC1200.021.5610391
TGGACGA403.9542792E-621.5610393
GTGGACG403.9542792E-621.5610392
GAGGACG17150.021.5610372
AGAGGAC17200.021.4983631
GATGGGT303.8603463E-420.9093210
GATGGCG354.463674E-520.9093210
ATGGGGG250.003348696320.9093211
GGGTTTT701.2732926E-1120.9093218
GGCGTTT303.8603463E-420.9093217
TTTCTGT354.463674E-520.9093221
GATGCTG303.8603463E-420.9093210
GATGTGG250.003348696320.9093210
GGATTTT303.8603463E-420.9093218
GATGTAG354.463674E-520.9093210
GATCGGT303.8603463E-420.9093210
GATCGGA303.8603463E-420.9093210
Produced by FastQC (version 0.11.2)