FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_26.3510000006087b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_26.3510000006087b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23227
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC3131.3475696387824514No Hit
ACTGTCTCTGTCTCTTATACACATCTC2010.8653721961510312No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1200.5166401171050933No Hit
ACTGTCTGTCTCTTATACACATCTCCG350.15068670082231886No Hit
ACTGTCTGTGTCCCTGTCTCTTATACA260.11193869203943686No Hit
ACTGTCTGTGTCGGGGCTGTCTCTTAT250.10763335773022774No Hit
ACTGTCTGTGTCTGTCTCTTATACACA240.10332802342101863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCT303.2818317E-421.4370861
CAGTCTG303.2818317E-421.4370862
ACGGTCT607.075869E-1021.4370861
ACTGCCT404.2117754E-621.4370841
ACCGTCT404.2117754E-621.4370841
CCGTCTG404.2117754E-621.4370842
CTGCCTG404.2117754E-621.4370842
CTGTCTG20300.021.3842872
ACTGTCT20800.021.0763661
GTGTCGT1850.020.928888
GTGTCAC1050.020.928888
TGTCTTG558.212737E-920.928889
TGTCTGG558.212737E-920.928889
AGGGTTT558.212737E-920.9288817
GAGTTTT558.212737E-920.9288818
CTGTGTC22650.020.928886
CGGTTTT558.212737E-920.9288818
TGTCGTC558.212737E-920.928889
TTGTTTT507.041672E-820.92887918
TGGTTTT354.473728E-520.92887918
Produced by FastQC (version 0.11.2)