FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_22.351000000607e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_22.351000000607e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42639
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCGCTGTCTCTTATACA1200.28143249138112997No Hit
GTCAGAACTACCCCTGTCTCTTATACA1160.2720514083350923No Hit
GTCAGAACTACCTCTGTCTCTTATACA530.12429935035999906No Hit
GTCAGAACTACCGGGGGGCTGTCTCTT470.11022772579094256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACT39100.021.1921733
TTAGAAC506.481969E-821.1921712
TAGAACT506.481969E-821.1921713
GTCCGAA1950.021.1921711
TAAGAAC250.003155596821.1921712
TCAGAAC38350.021.1921712
GCAGAAC455.5796227E-721.1921712
GTAAGAA250.003155596821.1921711
AAGAACT250.003155596821.1921713
GTTAGAA506.481969E-821.1921711
TCCGAAC1950.021.1921712
GTCAGAA38350.021.1921711
GGCAGAA455.5796227E-721.1921711
ACCGGTG507.256858E-820.96831110
ACCGGGC507.256858E-820.96831110
GGGTCTG250.003356617620.96831118
GGCATTT250.003356617620.96831117
ACCACGG558.532879E-920.96831110
GTTCTGT250.003356617620.96831120
GATGTTT507.256858E-820.96831117
Produced by FastQC (version 0.11.2)