FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_20.351000000607bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_20.351000000607bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24541
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT2440.9942545128560367No Hit
AGTCTCCTACGTCTGTCTCTTATACAC1870.761990138951143No Hit
AGTCTCCTACCTGTCTCTTATCCACAT880.3585835948005379No Hit
AGTCTCCTACGTCTGTCTCTTCTACAC720.2933865775640765No Hit
AGTCTCCTACGCTGTCTCTTATACACA540.2200399331730573No Hit
AGTCTCCTACGTGGCTGTCTCTTATAC280.11409478016380752No Hit
AGTCTCCTACGTGGGGGGCTGTCTCTT250.10187033943197099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCC250.002903478521.4518831
GCCTCCT250.002903478521.4518832
ACTCCTA250.002903478521.4518833
CCTCCTA250.002903478521.4518833
GTCTCCT22300.021.4518832
GGCTCCT454.7784306E-721.4518832
AGGCTCC454.7784306E-721.4518831
TCTCCTA22450.021.4518833
GCTCCTA454.7784306E-721.4518833
AGACTCC250.002903478521.4518831
GACTCCT250.002903478521.4518832
AGTCTCC22250.021.4518811
ACGTCTG651.1277734E-1020.9265339
TACGTAC651.1277734E-1020.9265338
CTACGTC5200.020.9265337
GGGTTTT1500.020.9265318
AGCTTTT250.003355016220.9265318
CCTACGT23950.020.926536
TTACTTT250.003355016220.9265317
AATTTTT609.677024E-1020.9265319
Produced by FastQC (version 0.11.2)