FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_2.3510000006057e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_2.3510000006057e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33293
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA560.1682035262667828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTG28750.021.2378061
GAGTGAG800.021.2378043
GTCGTGA1550.021.2378042
GAAGTGA354.061759E-521.2378042
TAATGAG404.6724144E-621.2378043
TCGTGAG1550.021.2378043
GGGAGTG800.021.2378041
GGAAGTG354.061759E-521.2378041
AAGTGAG354.061759E-521.2378043
GTAATGA404.6724144E-621.2378042
TTAGTGA303.5424894E-421.2378042
GTAGTGA28700.021.2378042
GGAGTGA800.021.2378042
GGTCGTG1550.021.2378041
CAGTGAG557.208655E-921.2378033
TAGTGAG29050.021.1646983
CCTTTTT1150.020.9819319
TGGTTTT558.300049E-920.98192818
ACGCTTT250.003328624920.98192817
CAGTTTT303.8461178E-420.98192818
Produced by FastQC (version 0.11.2)