FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_18.3510000006077c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_18.3510000006077c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14078
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA950.674811763034522No Hit
GCATTGGATACCGGCTGTCTCTTATAC600.4261969029691718No Hit
GCATTGGATACCCTGTCTCTTATACAC400.28413126864611454No Hit
GCATTGGATACCGCCTGTCTCTTATAC370.2628214234976559No Hit
GCATTGGATACCTGCTGTCTCTTATAC370.2628214234976559No Hit
GCATTGGATACCAGCTGTCTCTTATAC370.2628214234976559No Hit
GCATTGGATACCGTCTGTCTCTTATAC360.25571814178150304No Hit
GCATTGGATACCCCCTGTCTCTTATAC330.2344082966330445No Hit
GCATTGGATACCATCTGTCTCTTATAC260.18468532461997442No Hit
GCATTGGATACCCGCTGTCTCTTATAC230.16337547947151584No Hit
GCATTGGATACCGACTGTCTCTTATAC220.156272197755363No Hit
GCATTGGATACCCTCTGTCTCTTATAC170.12075578917459867No Hit
GCATTGGATACCCTGTCTCTTCTACAC170.12075578917459867No Hit
GCATTGGATACCCCTGTCTCTTATACA160.1136525074584458No Hit
GCATTGGATACCTCCTGTCTCTTATAC150.10654922574229295No Hit
GCATTGGATACCGCTGTCTCTTATACA150.10654922574229295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGGAT454.5983325E-721.3927293
CTTTGGA454.5983325E-721.3927292
GCATTGG12250.021.3927291
GCTTTGG454.5983325E-721.3927291
GCCTTGG657.2759576E-1121.3927271
CCTTGGA657.2759576E-1121.3927272
CTTGGAT657.2759576E-1121.3927273
ATTGGAT12500.021.3071563
CATTGGA12350.021.3061182
ACCGGCT404.940952E-620.93594410
AATTTTT800.020.93594419
GATACCT2550.020.9359447
GATACCG5150.020.9359447
GATACCC3000.020.9359447
GATACCA3150.020.9359447
TGCTGTC455.6790304E-720.93594419
GGATACC13800.020.9359446
TACCGGT455.6790304E-720.9359449
TACCGGC850.020.9359449
TACCGCT557.501512E-920.9359449
Produced by FastQC (version 0.11.2)