FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_16.3510000006072c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_16.3510000006072c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30727
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC2420.787580954860546No Hit
GAACACCATAGCGGCTGTCTCTTATAC1950.6346210173463078No Hit
GAACACCATAGCGCCTGTCTCTTATAC1030.3352100758290754No Hit
GAACACCATAGCCTGTCTCTTCTACAC1020.33195560907345334No Hit
GAACACCATAGCAGCTGTCTCTTATAC890.2896475412503661No Hit
GAACACCATAGCACCTGTCTCTTATAC720.23432160640479058No Hit
GAACACCATAGCCCCTGTCTCTTATAC710.23106713964916847No Hit
GAACACCATAGCGTCTGTCTCTTATAC620.20177693884856968No Hit
GAACACCATAGCTGCTGTCTCTTATAC600.1952680053373255No Hit
GAACACCATAGCATCTGTCTCTTATAC540.17574120480359293No Hit
GAACACCATAGCGACTGTCTCTTATAC500.16272333778110457No Hit
GAACACCATAGCCCTGTCTCTTATACA480.15621440426986039No Hit
GAACACCATAGCTGTCTCTTATACACA450.14645100400299413No Hit
GAACACCATAGCCGCTGTCTCTTATAC440.14319653724737202No Hit
GAACACCATAGCTCCTGTCTCTTATAC430.13994207049174992No Hit
GAACACCATAGCTTCTGTCTCTTATAC350.11390633644677318No Hit
GAACACCATAGCTACTGTCTCTTATAC320.10414293617990691No Hit
GAACACCATAGCCTCTGTCTCTTATAC320.10414293617990691No Hit
GAACACCATAGCAACTGTCTCTTATAC310.10088846942428484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACCA1300.021.328362
GTACACC850.021.328361
AACCCCA1300.021.328362
TACACCA850.021.328362
CCACCAT1300.021.328363
GAACCCC1300.021.328361
ACCCCAT1300.021.328363
GACCACC1300.021.328361
ACACCAT26800.021.3283583
GAACACC25950.021.3283581
GATCACC556.8084773E-921.3283581
ATCACCA556.8084773E-921.3283582
TCACCAT556.8084773E-921.3283583
AACACCA25950.021.3283582
TGGTTTT800.020.94625518
TAGCGGG1600.020.9462559
TAGCGCG1350.020.9462559
TAGCGAG950.020.9462559
AATTTTT800.020.94625519
ACCATAG29250.020.9462555
Produced by FastQC (version 0.11.2)