FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_15.35100000060702.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_15.35100000060702.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15397
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA1701.1041111904916543No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT570.3702019874001429No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT320.20783269468078197No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC320.20783269468078197No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT310.20133792297200753No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT270.17535883613690978No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA240.1558745210105865No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA220.1428849775930376No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT210.13639020588426318No Hit
TGGCATAGCTGAGGAGCTGTCTCTTAT200.12989543417548874No Hit
TGGCATAGCTGAGGGTCTGTCTCTTAT180.11690589075793985No Hit
TGGCATAGCTGAAGGGGCTGTCTCTTA160.10391634734039099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCATA250.002873088221.4266661
TGGCATA13150.021.4266661
TGTCATA353.6189012E-521.4266661
TGGCCTA950.021.4266661
TGCATAG250.002929577621.3554822
GTCATAG353.714125E-521.3554822
GGCATAG13200.021.3554822
GCCTAGC950.021.3554823
GGCCTAG950.021.3554822
TCATAGC353.714125E-521.3554823
GCATAGC13450.021.3554823
GTGTTTT455.745951E-720.93811218
CTGAGTG651.0186341E-1020.9381129
CTGAGTC455.745951E-720.9381129
CTGAGGA651.0186341E-1020.9381129
CTGAGAG651.0186341E-1020.9381129
AATTTTT455.745951E-720.93811219
TGTTTTT850.020.93811219
TAGCTGC455.745951E-720.9381126
GTTTTTT4850.020.93811220
Produced by FastQC (version 0.11.2)