FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_13.351000000606da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_13.351000000606da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTGGCTGTCTCTTATACA950.677796803652968No Hit
GACTCTATGGTCTGTCTCTTATACACA910.6492579908675798No Hit
GACTCTATGGTGCCTGTCTCTTATACA670.4780251141552511No Hit
GACTCTATGGTGTCTGTCTCTTATACA400.28538812785388123No Hit
GACTCTATGGTGACTGTCTCTTATACA300.21404109589041095No Hit
GACTCTATGGTGCTGTCTCTTATACAC240.17123287671232876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATG900.021.260873
ACTCTAT11850.021.260872
GATTCTA353.8133963E-521.260871
TTCTATG353.8133963E-521.260873
ATTCTAT353.8133963E-521.260872
GACTCTA11850.021.260871
GACTATA900.021.260871
ACTATAT900.021.260872
CTCTATG12050.021.2608683
GGTGATG455.6209683E-720.9571449
GGGTTTT506.45432E-820.95714418
GGTGAGG250.003256394520.9571449
TATATGG900.020.9571444
AATTTTT557.408744E-920.95714419
CCGCTTT250.003256394520.95714417
GTTCTGT250.003256394520.95714420
ATGGTGT3500.020.9571447
ATGGTGG5000.020.9571447
ATGGTGA2500.020.9571447
GTGGGAG250.003256394520.95714410
Produced by FastQC (version 0.11.2)