FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_12.351000000606b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_12.351000000606b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22893
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1380.6028043506748788No Hit
GAATCCTTACGCCTGTCTCTTCTACAC370.16162145634036604No Hit
GAATCCTTACGCTGTCTCTTATACACA250.10920368671646355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTA751.8189894E-1221.3207133
ACTCCTT751.8189894E-1221.3207132
GACTCCT751.8189894E-1221.3207131
AATCCTT19500.021.3207112
TTCCTTA800.021.3207113
TATCCTT455.040456E-721.3207112
GAATCCT19500.021.3207111
GATTCCT800.021.3207111
AATACTT900.021.3207112
GAATACT900.021.3207111
ATACTTA900.021.3207113
ATTCCTT800.021.3207112
ATCCTTA19950.021.3207113
TCCTTTT354.4393797E-520.94748518
ACGCCTA250.003328768820.9474859
ACGCTTA303.8377597E-420.9474859
CAGTTTT354.4393797E-520.94748518
GGCATTT303.8377597E-420.94748517
ACGCCGT303.8377597E-420.9474859
ACGCCGA303.8377597E-420.9474859
Produced by FastQC (version 0.11.2)