FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_10.3510000006067d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_10.3510000006067d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21776
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC2090.9597722263041881No Hit
ACGTCTCTGCCTGTCTCTTATACACAT960.440852314474651No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC900.41329904481998525No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT390.17909625275532695No Hit
ACGTCTCTGCATGGGGGCTGTCTCTTA230.1056208670095518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC19200.021.2517491
ACGTATC1550.021.2517491
CGTATCT1550.021.2517492
CGTCTCT19200.021.2517492
GTCTCTG19450.021.2517473
ATGGGGA250.003313415220.95862211
TGCATTC651.09139364E-1020.9586228
TGCATTA850.020.9586228
TGCATGC2050.020.9586228
TGCATGA1700.020.9586228
CAGTTTT506.885966E-820.95862218
TGCATCT1300.020.9586228
TGCATCG1700.020.9586228
TGCATCC850.020.9586228
CATGAGT250.003313415220.95862210
CATGAGG250.003313415220.95862210
CATGAGC250.003313415220.95862210
TGCATAA1000.020.9586228
AAATTTT250.003313415220.95862218
ACGTTTT354.4085267E-520.95862218
Produced by FastQC (version 0.11.2)