FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_93.351000000604dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_93.351000000604dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24343
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTCTGTCTCTTATACACA260.10680688493612125No Hit
ATCTCGACGCTGTCTCTTATACACATC250.10269892782319352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG20900.021.3012563
ATCTCGA20150.021.3012561
AGCTCGA455.1137977E-721.3012561
TCTAGAC1700.021.3012562
ATCTAGA1700.021.3012561
TATCGAC505.862239E-821.3012562
TTCGACG250.00302465821.3012563
TCTCGAC20150.021.3012562
GCTCGAC455.1137977E-721.3012562
CTAGACG1700.021.3012563
ATTTCGA250.00302465821.3012561
ATCGACG505.862239E-821.3012563
TTTCGAC250.00302465821.3012562
GTGTTTT558.163624E-920.95061918
GGGTTTT1100.020.95061918
CAGTTTT354.4493296E-520.95061918
ACGCTAC4750.020.9506197
TAGTGGG250.003331931520.95061911
GCTAGTC701.2732926E-1120.9506199
AATTTTT1100.020.95061919
Produced by FastQC (version 0.11.2)