FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_85.351000000603c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_85.351000000603c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30400
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1480.4868421052631579No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC370.12171052631578948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTGT303.4960345E-421.2704521
CTCTAGT26550.021.2704521
TCTTGTA303.4960345E-421.2704522
CTTGTAC303.4960345E-421.2704523
CTTTAGT250.00306815821.270451
GCTAGTA250.00306815821.270452
CTCTCGT1550.021.270451
CGCTAGT250.00306815821.270451
TCTCGTA1550.021.270452
TTTAGTA250.00306815821.270452
CTAGTAC27450.021.270453
CACTAGT250.00306815821.270451
ACTAGTA250.00306815821.270452
TTAGTAC250.00306815821.270453
CTCGTAC1550.021.270453
TCTAGTA26650.021.2704492
CCTAGTA353.9988583E-521.2704492
CCCTAGT353.9988583E-521.2704491
GAGGTGG250.003346500220.95559311
TCCTTTT250.003346500220.95559318
Produced by FastQC (version 0.11.2)