FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_83.3510000006038d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_83.3510000006038d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55203
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA5260.9528467655743347No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA660.11955871963480245No Hit
CACAGAAGGACTGCTGTCTCTTATACA580.10506675361846277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAAG250.00317706321.1811542
CACAAAA250.00317706321.1811541
CAAAAGG250.00317706321.1811543
TAGAAGG900.021.1811523
ACAGAAG48900.021.1811522
CACCGAA3500.021.1811521
ACCGAAG3500.021.1811522
TCAGAAG850.021.1811522
CATAGAA900.021.1811521
CAGAAGG49700.021.1811523
CACAGAA48800.021.1811521
CCGAAGG3500.021.1811523
CTTCAGC250.003367427520.9701111
ACTGCGT558.6656655E-920.9701110
ACTGCGC250.003367427520.9701110
ACTGTGA250.003367427520.9701110
ACTCGTA250.003367427520.9701110
GTGTTTT1000.020.9701118
ACTAACC250.003367427520.9701110
ACTCGGC250.003367427520.9701110
Produced by FastQC (version 0.11.2)