FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_76.3510000006029b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_76.3510000006029b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30135
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC2670.8860129417620707No Hit
AGAGGACGAGCTGTCTCTTATACACAT1730.5740832918533267No Hit
AGAGGACGAGATCTGTCTCTTCTACAC870.2887008461921354No Hit
AGAGGACGAGATGCTGTCTCTTATACA680.22565123610419777No Hit
AGAGGACGAGCTGTCTCTTATCCACAT570.18914883026381285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGACG25150.021.4285722
AGAGGAC25100.021.4285721
AGTGGAC607.385097E-1021.4285721
TGGACGA607.385097E-1021.4285723
GTGGACG607.385097E-1021.4285722
GAGAACG353.773754E-521.428572
AGGACGA25250.021.428573
CGGACGA2750.021.428573
GCGGACG2750.021.428572
AGAACGA353.773754E-521.428573
AGCGGAC2750.021.428571
AGAGAAC353.773754E-521.428571
ATGGGTG456.165574E-720.93023311
GATGGGG558.458301E-920.93023310
GATGGGC250.00336931220.93023310
GATGGGA250.00336931220.93023310
GATGGCG303.8989578E-420.93023310
GATGGAA456.165574E-720.93023310
ATGGGGG456.165574E-720.93023311
GTGTTTT609.931682E-1020.93023318
Produced by FastQC (version 0.11.2)