FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_73.3510000006023e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_73.3510000006023e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36516
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC890.24372877642677182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTCG404.526428E-621.326363
TAGGTTC404.526428E-621.326362
GGGTTCG1050.021.3263593
CTGGGTT1050.021.3263591
TCGGTTC303.4529757E-421.3263592
TGATTCG303.4529757E-421.3263593
CTTGGTT32450.021.3263591
CTGGTTC250.003033352821.3263592
TTGATTC303.4529757E-421.3263592
TGGGTTC1050.021.3263592
GTGGTTC505.999573E-821.3263592
CGGTTCG303.4529757E-421.3263593
CCTGGTT250.003033352821.3263591
TGGTTCG33550.021.2945773
TTGGTTC32700.021.2285332
AACGTTT303.9004645E-420.94657717
TGCATTT303.9004645E-420.94657717
GTGTTTT1550.020.94657718
GTTGAGC601.0040822E-920.9465779
GTTGACG751.8189894E-1220.9465779
Produced by FastQC (version 0.11.2)