FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_71.351000000601f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_71.351000000601f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50203
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA3000.5975738501683167No Hit
ATCTGCTATCCTGTCTCTTATACACAT890.1772802422166006No Hit
ATCTGCTATCCGGCTGTCTCTTATACA630.1254905085353465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCT455.0645576E-721.422021
CTCCTAT455.0645576E-721.422023
TATGCTA455.0645576E-721.422022
ATATGCT455.0645576E-721.422021
TCTCCTA455.0645576E-721.422022
TTTGCTA701.0913936E-1121.422022
TTGCTAT701.0913936E-1121.422023
CCTGCTA303.3746322E-421.4220182
TCTACTA751.8189894E-1221.4220182
TCTGCTA45650.021.4220182
CTGCTAT46600.021.4220183
ATCTGCT45650.021.4220181
CTACTAT751.8189894E-1221.4220183
AGGGGGT354.605993E-520.93127615
CCGGAGT354.605993E-520.93127610
CCGTGAG354.605993E-520.93127610
GCGATTT354.605993E-520.93127617
GGCGTTT651.2369128E-1020.93127617
TTTCTGT651.2369128E-1020.93127621
GAAGTTT354.605993E-520.93127617
Produced by FastQC (version 0.11.2)