FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_69.351000000601c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_69.351000000601c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47307
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC770.1627666095926607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAAC751.8189894E-1221.408653
CATTGAA659.094947E-1121.408652
ATCTTGA455.08202E-721.408651
CCTTGAA43900.021.408652
ACATTGA659.094947E-1121.408651
ACTTTGA751.8189894E-1221.408651
TCTTGAA455.08202E-721.408652
ATTGAAC659.094947E-1121.408653
ACCTTGA43850.021.408651
CTAGAAC303.3848415E-421.408653
CTTTGAA751.8189894E-1221.408652
CCTAGAA303.3848415E-421.408652
CTTGAAC44350.021.4086483
TGGTCGC250.003394681320.93340510
TGGCATA354.595134E-520.93340510
TGGTTAG250.003394681320.93340510
CAGTTTT558.760253E-920.93340518
AAATTTT250.003394681320.93340518
TGGTATA250.003394681320.93340510
ACGTTTT405.3778604E-620.93340518
Produced by FastQC (version 0.11.2)