FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_68.351000000601a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_68.351000000601a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48560
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT4250.8752059308072487No Hit
AGTCTCCTACGTCTGTCTCTTATACAC3780.7784184514003295No Hit
AGTCTCCTACGCTGTCTCTTATACACA1040.214168039538715No Hit
AGTCTCCTACGTGGCTGTCTCTTATAC550.11326194398682042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCTA658.731149E-1121.4561793
AGACTCC658.731149E-1121.4561791
GACTCCT658.731149E-1121.4561792
AGCCTCC505.7241778E-821.4561771
GCCTCCT505.7241778E-821.4561772
GTATCCT607.6033757E-1021.4561772
AGTATCC607.6033757E-1021.4561771
CCTCCTA505.7241778E-821.4561773
TATCCTA607.6033757E-1021.4561773
TTCTCCT607.6033757E-1021.4561772
AGGCTCC1100.021.4561771
TCACCTA607.6033757E-1021.4561773
TCTCCTA43600.021.4561773
GCTCCTA1100.021.4561773
AGTCCCC250.002942216621.4561771
ATTCTCC607.6033757E-1021.4561771
AGTCTCC43000.021.4561771
AGTCACC607.6033757E-1021.4561771
TCCCCTA250.002942216621.4561773
GTCTCCT43050.021.4312572
Produced by FastQC (version 0.11.2)