FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_63.35100000060108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_63.35100000060108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41913
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA3210.7658721637678048No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT1520.3626559778588982No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT670.15985493760885644No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT600.14315367547061772No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC560.13361009710590985No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT550.1312242025147329No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA520.124066518741202No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT440.10497936201178631No Hit
TGGCATAGCTGATGGGCTGTCTCTTAT440.10497936201178631No Hit
TGGCATAGCTGACGGGCTGTCTCTTAT430.10259346742060936No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA420.1002075728294324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCATA353.8674592E-521.3951231
TGCATAG353.8674592E-521.3951232
GCCTAGC2600.021.3951233
GGCCTAG2600.021.3951232
TGGAATA250.002984481421.3951231
TGGCGTA250.002984481421.3951231
GAATAGC250.002984481421.3951233
GCGTAGC250.002984481421.3951233
GGAATAG250.002984481421.3951232
TGGCCTA2600.021.3951231
TGGCATA36850.021.395121
GTCATAG303.3902007E-421.395122
GGCATAG36850.021.395122
TGTCATA303.3902007E-421.395121
GGCTTAG303.3902007E-421.395122
TCATAGC303.3902007E-421.395123
TGGCTTA303.3902007E-421.395121
GCATAGC37150.021.395123
GCTTAGC303.3902007E-421.395123
TGATGGG800.020.93556210
Produced by FastQC (version 0.11.2)