FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_60.351000000600aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_60.351000000600aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43884
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC2300.5241090146750524No Hit
GAATCCTTACGCTGTCTCTTATACACA630.1435602953240361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTT37700.021.2920062
CTCCTTA1500.021.2920063
ACTCCTT1500.021.2920062
TTCCTTA900.021.2920063
GAATCCT37650.021.2920061
GATTCCT900.021.2920061
AATACTT1500.021.2920062
GAATACT1500.021.2920061
ATACTTA1500.021.2920063
ATTCCTT900.021.2920062
GACTCCT1500.021.2920061
ATCCTTA38850.021.2920043
CGCTGCC354.553819E-520.9521110
GGGTTTT2500.020.9521118
ACGCCTT601.0168151E-920.952119
ACGCCTG1500.020.952119
ACGCTTC303.9108933E-420.952119
ACGCTTA354.553819E-520.952119
GCCTTTT701.4551915E-1120.9521118
ACGCCGC950.020.952119
Produced by FastQC (version 0.11.2)