FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_58.35100000060066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_58.35100000060066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62506
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC4550.7279301187086039No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1700.2719738905065114No Hit
ACGTCTCTGCATGGGGGCTGTCTCTTA670.10718970978786037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC55300.021.2497981
ACGTATC4100.021.2497981
ACATCTC303.5782185E-421.2497981
ACTTCTC303.5782185E-421.2497981
ACGTTTC404.7612393E-621.2497961
GTCTCTG56000.021.2325783
ATCTCTG303.597914E-421.2325763
GTATCTG4100.021.2325763
TTCTCTG303.597914E-421.2325763
CGTATCT4100.021.2325762
CTTCTCT303.597914E-421.2325762
CGTCTCT55250.021.2325762
CGTCATT250.00338105620.96080216
CATGATA250.00338105620.96080210
ATGGGAG507.4236596E-820.96080211
CATGACC250.00338105620.96080210
AGCTTTT507.4236596E-820.96080218
GGGCATT250.00338105620.96080216
CATGAAG250.00338105620.96080210
ACGCTGT250.00338105620.96080218
Produced by FastQC (version 0.11.2)