FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_57.3510000006004d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_57.3510000006004d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34545
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA3751.0855405992184108No Hit
TACGGTACGTAGCCTGTCTCTTATACA1650.4776378636561007No Hit
TACGGTACGTAGTCTGTCTCTTATACA1190.3444782168186423No Hit
TACGGTACGTAGACTGTCTCTTATACA1090.3155304675061514No Hit
TACGGTACGTAGCTGTCTCTTATACAC1040.30105659284990594No Hit
TACGGTACGTAGGGCTGTCTCTTATAC810.2344767694311767No Hit
TACGGTACGTAGGGGCTGTCTCTTATA780.22579244463742945No Hit
TACGGTACGTAGAGCTGTCTCTTATAC490.14184397163120568No Hit
TACGGTACGTAGGCCTGTCTCTTATAC440.1273700969749602No Hit
TACGGTACGTAGGGCCTGTCTCTTATA420.12158054711246201No Hit
TACGGTACGTAGCTGTCTCTTCTACAC370.10710667245621654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC607.4942363E-1021.4273012
TTCGGTA607.4942363E-1021.4273011
CGATACG658.54925E-1121.42733
TGGTACG505.6814315E-821.42733
TACGGTA31550.021.42731
ATGGTAC505.6814315E-821.42732
TACGATA658.54925E-1121.42731
ACGATAC658.54925E-1121.42732
ACGGTAC31550.021.42732
CGGTACG32150.021.42733
TATGGTA505.6814315E-821.42731
GTAGCGG850.020.9304379
GTACGTA33700.020.9304375
GTAGATT507.2939656E-820.9304359
GTAGATG303.91478E-420.9304359
TGGTCTG250.003379047620.93043518
TGGTTTT354.5537556E-520.93043518
TAGCGCG354.5537556E-520.93043510
GTAGAGT354.5537556E-520.9304359
GTAGAGA507.2939656E-820.9304359
Produced by FastQC (version 0.11.2)