FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_56.35100000060023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_56.35100000060023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35463
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC7942.2389532752446213No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC2540.7162394608465161No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC1800.5075712714660351No Hit
ACAGTGCTCATGGCTGTCTCTTATACA920.25942531652708456No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC690.19456898739531342No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC670.18892930660124638No Hit
ACCGTGCTCATGCTGTCTCTTCTACAC580.16355074302794462No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC500.1409920198516764No Hit
ACAGTGCTCCTGTCTCTTATACACATC440.12407297746947522No Hit
ACAGTGCTCATGTGCTGTCTCTTATAC420.11843329667540817No Hit
ACAGTGCTCATGACCTGTCTCTTATAC360.10151425429320701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC250.003001784221.362591
ACTGTGC250.003001784221.362591
ACAGAGC250.003001784221.362591
AGAGCTC250.003001784221.362593
AGCGCTC250.003001784221.362593
CAGCGCT250.003001784221.362592
CAGTGCT30950.021.362592
TGTGCTC250.003001784221.362593
CCGTGCT2750.021.3625892
ACCGTGC2750.021.3625891
CGTGCTC2750.021.3625893
AGTGCTC31200.021.3283563
ACAGTGC31000.021.3281331
TCCTTTT405.2989344E-620.94076318
ATGGGCA405.2989344E-620.94076310
AGCTTTT405.2989344E-620.94076318
CATGAAG405.2989344E-620.9407639
ATGGCGG558.540155E-920.94076310
GGTTCTG405.2989344E-620.94076319
TAGTTTT558.540155E-920.94076318
Produced by FastQC (version 0.11.2)