FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_55.3510000005fffd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_55.3510000005fffd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52441
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1870.3565912167960184No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT650.12394881867241281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC404.6801724E-621.279071
ACAGCAA404.6801724E-621.279073
CTTCAGC43400.021.279071
CGTCAGC557.2923285E-921.279071
TACAGCA404.6801724E-621.279072
CCAGCAA701.0913936E-1121.279073
TCGGCAA455.412876E-721.279073
CTCCAGC701.0913936E-1121.279071
TTCTGCA404.6801724E-621.279072
CATCAGC303.5342557E-421.279071
TCCAGCA701.0913936E-1121.279072
ATCAGCA303.5342557E-421.279072
TCAGCAA44750.021.279073
TCTGCAA404.6801724E-621.279073
GCAGCAA1250.021.279073
CTCAGCA506.276423E-821.279072
CCTCAGC506.276423E-821.279071
CTGCAGC1250.021.279071
TTCAGCA43400.021.279072
GTCAGCA557.2923285E-921.279072
Produced by FastQC (version 0.11.2)