FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_54.3510000005ffd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_54.3510000005ffd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38228
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA4001.0463534581981793No Hit
CTGATCGTAGCACCTGTCTCTTATACA1440.3766872449513446No Hit
CTGATCGTAGCAGCTGTCTCTTATACA800.20927069163963588No Hit
CTGATCGTAGCATCTGTCTCTTATACA550.14387360050224968No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT480.1255624149837815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT250.003151084521.1904772
GATCGTA35350.021.1904773
GGATCGT651.0186341E-1021.1904772
CGGATCG651.0186341E-1021.1904771
CTTATCG250.003151084521.1904771
TGATCGT34200.021.1904752
CTGATCG34200.021.1904751
GCTCGTA1900.021.1904753
TGCTCGT1900.021.1904752
CTGCTCG1900.021.1904751
CCGATCG404.7957274E-621.1904751
CGATCGT404.7957274E-621.1904752
GTCATTT250.003350074620.96858817
GGCATTT250.003350074620.96858817
CAGTTGG250.003350074620.96858811
GCAAGGC250.003350074620.96858810
TATACAC507.2061084E-820.96858820
GTCGTTT250.003350074620.96858817
TTTCTGT850.020.96858821
GGTCTTT250.003350074620.96858817
Produced by FastQC (version 0.11.2)