FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_52.3510000005ff90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_52.3510000005ff90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23294
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC3021.296471194298961No Hit
AGACGTCGATACCTGTCTCTTCTACAC920.3949514896539882No Hit
AGACGTCGATACGGGGGGGGTTTTTTT280.1202026272859964No Hit
AGACGTCGATACCCTGTCTCTTATACA270.11590967631149651No Hit
AGACGTCGATACACCTGTCTCTTATAC270.11590967631149651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA20100.021.4768223
AGACGTC19900.021.4768221
GACGTCG19900.021.4768222
ACCTCGA303.241004E-421.4768223
AGACCTC303.241004E-421.4768221
GACCTCG303.241004E-421.4768222
GTCGTCG404.1446765E-621.476822
CCGTCGA1650.021.476823
AGTCGTC404.1446765E-621.476821
TCGTCGA404.1446765E-621.476823
GCCGTCG1650.021.476822
AGCCGTC1650.021.476821
TGGTTTT651.1277734E-1020.9225818
GTGTTTT651.1277734E-1020.9225818
GGGTTTT1150.020.9225818
GCCTTTT303.871555E-420.9225818
CAGTTTT354.4846627E-520.9225818
GGCATTT303.871555E-420.9225817
AGCTTTT456.062219E-720.9225818
CGATACT4450.020.922587
Produced by FastQC (version 0.11.2)