FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_50.3510000005ff5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_50.3510000005ff5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32964
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA560.16988229583788375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGA1900.021.2773512
GTACTGA303.497123E-421.2773512
TCGTGAG1900.021.2773513
GGTACTG303.497123E-421.2773511
GGTCGTG1900.021.2773511
GAAGTGA506.1018E-821.277352
GGGAGTG250.003067873721.277351
GCAGTGA354.002198E-521.277352
GGAAGTG506.1018E-821.277351
CAGTGAG354.002198E-521.277353
GTAGTGA28750.021.277352
GGAGTGA250.003067873721.277352
TAGTGAG29000.021.2406643
GGTAGTG28800.021.240411
AGCTAAG2750.020.954488
TAGCGGG303.8794105E-420.95447711
GTGTTTT751.8189894E-1220.95447718
GGGTTTT1450.020.95447718
GGCATTT303.8794105E-420.95447717
CTGAGCT303.8794105E-420.9544775
Produced by FastQC (version 0.11.2)