FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_5.3510000005f9cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_5.3510000005f9cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTACACCTGTCTCTTATA60.48No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT50.4No Hit
GAGCTACGTCTGTCTCTTATACACATC50.4No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC50.4No Hit
GAGCTACGTCTATGCTGTCTCTTATAC40.32No Hit
GAGCTACGTCTAAGCTGTCTCTTATAC40.32No Hit
GAGCTACGTCTAACGGCTGTCTCTTAT30.24No Hit
GAGCTACGTCTATGGGCTGTCTCTTAT30.24No Hit
GAGCTACGTCTATGACCTGTCTCTTAT30.24No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA30.24No Hit
GAGCTACGTCTAGACCTGTCTCTTATA30.24No Hit
GAGCTACGTCTAGGCGGGTGTTTTTTT30.24No Hit
GAGCTACGTCTAGAAACTGTCTCTTAT30.24No Hit
GAGCTACGTCTACTGTCTGTCTCTTAT20.16No Hit
GAGCTACGTCTATTTTCTCTGTCTCTT20.16No Hit
GAGCTACGTCTAAGGGCTGTCTCTTAT20.16No Hit
GAGCTACGTCTATTCTGTCTCTTATAC20.16No Hit
GAGCTACGTCTATGTCAGCGTTTTTTT20.16No Hit
GAGCTACGTCTAGGCGTCTCTTTTTTT20.16No Hit
GAGCTACGTCTAGGTTGGGGTTTTTTT20.16No Hit
GAGCTACGTCTAGCGGAGGCTTTTTTT20.16No Hit
GAGCTACGTCTAAGACCTGTCTCTTAT20.16No Hit
GAGCTACGTCTATAGCGGGATTTTTTT20.16No Hit
GAGCTACGTCTACTAGAGTATTTTTTT20.16No Hit
GAGCTACGTCTAGCTGTCTCTTATACA20.16No Hit
GAGCTACGTCTAGGCATGCCTTTTTTT20.16No Hit
GAGCTACGTCTAGGCCTGTCTCTTATA20.16No Hit
GAGCTACGTCTATGGTCTGTCTCTTAT20.16No Hit
GAGCTACGTCTAGGTCTGTCTCTTATA20.16No Hit
GAGCTACGTCTGTCTCTTATAAACATC20.16No Hit
GAGCTACGTCTACCTGTCTCTTATACA20.16No Hit
GAGCTACGTCTAGAGCTGTCTCTTATA20.16No Hit
GAGCTACGTCTAATTCGCTATTTTTTT20.16No Hit
GAGCTACGTCTAATGGCTGTCTCTTAT20.16No Hit
GAGCTACGTCTACTCTATGGCTGTCTC20.16No Hit
GAGCTACGTCTAGGAACTACTTTTTCT20.16No Hit
GAGCTACGTCTATTCCTGTCTCTTATA20.16No Hit
GAGCTACGTCTCTGTCTCTTATACACA20.16No Hit
GAGCTACGTCTAACCTGTCTCTTATAC20.16No Hit
GAGCTACGTCTAGGGCGTTCTTTTTTT20.16No Hit
GAGCTACGTCTAAGAGGGGGTTCTGTC20.16No Hit
GAGCTACGTCTACCTAGTTGTTTTTTT20.16No Hit
GAGCTACGTCTAAAGGCTGTCTCTTAT20.16No Hit
GAGCTACGTCTGTCTCTTATCCACCTC20.16No Hit
GAGCTACGTCTATACACGCGTTTTTTT20.16No Hit
GAGCTACGTCTATCCAAGAATTTTTTT20.16No Hit
GAGCTACGTCTAGCACCTGTCTCTTAT20.16No Hit
GAGCTACGTCTAGTGTGGTCTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACG1150.021.752
GAGCTAC1150.021.751
GCTACGT1150.021.753
ACGTCTA1250.020.8800016
TTTTTTT800.020.88000121
GTTTTTT401.2504061E-620.88000120
TACGTCT1250.020.8800015
CTACGTC1200.020.8800014
GTCTAGG351.5117206E-520.8800018
CGTCTAC250.001916530120.8800017
CGTCTAA250.001916530120.8800017
CGTCTAG555.0567905E-1020.887
Produced by FastQC (version 0.11.2)