FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_47.3510000005fefe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_47.3510000005fefe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGCTGTCTCTTATACA110.28335909325090164No Hit
TAGCAGACTGGAGGCTGTCTCTTATAC100.2575991756826378No Hit
TAGCAGACTGGACCTGTCTCTTATACA50.1287995878413189No Hit
TAGCAGACTGGATACTGTCTCTTATAC50.1287995878413189No Hit
TAGCAGACTGGATGCTGTCTCTTATAC40.10303967027305513No Hit
TAGCAGACTGGATAGGGGCTGTCTCTT40.10303967027305513No Hit
TAGCAGACTGGAGGGGAGGGTTTTTTT40.10303967027305513No Hit
TAGCAGACTGGCTGTCTCTTATACACA40.10303967027305513No Hit
TAGCAGACTGGAAGCTGTCTCTTATAC40.10303967027305513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGA3800.021.0000026
ACTGGAC750.021.0000027
TGGTTTT250.00281337121.018
TAGCCGA453.6522033E-721.01
GTTTTTT503.8086E-821.020
GGTTTTT303.0402135E-421.019
CATTTTT303.0402135E-421.019
TGGAGGG303.0402135E-421.09
TAGCAGA3250.021.01
AGCAGAC3250.021.02
CTGGATT303.0402135E-421.08
CTGGATG303.0402135E-421.08
CTGGAGG654.0017767E-1121.08
CTGGAGC303.0402135E-421.08
CTGGAGA503.8086E-821.08
CTGGACT250.00281337121.08
CTGGAAA250.00281337121.08
GCAGACT3250.021.03
CAGACTG3250.021.04
AGCCGAC453.6522033E-721.02
Produced by FastQC (version 0.11.2)