FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_41.3510000005fe34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_41.3510000005fe34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC311.2081060015588463No Hit
TCAACCGATAGGCTGTCTCTTCTACAC180.7014809041309431No Hit
TCAACCGATAGCTGTCTCTTATACACA60.23382696804364772No Hit
TCAACCGATAGGCCTGTCTCTTATACA60.23382696804364772No Hit
TCAACCGATAGGACCTGTCTCTTATAC50.19485580670303976No Hit
TCAACCGATAGGGCTGTCTCTTATACA50.19485580670303976No Hit
TCAACCGATAGGGGCTGTCTCTTATAC50.19485580670303976No Hit
TCAACCGATAGGGTCTGTCTCTTATAC40.1558846453624318No Hit
TCAACCGATAGGAGCTGTCTCTTATAC30.11691348402182386No Hit
TCAACCGATAGGGCCTGTCTCTTATAC30.11691348402182386No Hit
TCAACCGATAGGATCTGTCTCTTATAC30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT2350.021.03
CGATAGG2550.021.06
CAACCGA2350.021.02
ATAGGGT352.7053582E-521.08
ATAGGGA302.6474395E-421.08
TTTTTTT1550.021.021
GTTTTTT602.3101165E-1021.020
TCAACCG2350.021.01
ACCGATA2400.021.04
CCGATAG2500.021.05
GATAGGT652.1827873E-1121.07
GATAGGC352.7053582E-521.07
ATAGGGG250.002551460920.9999988
GGTTTTT250.002551460920.99999819
TCTGTCT250.002551460920.99999821
ATTTTTT502.602792E-820.99999820
GATAGGG1100.020.9999987
GATAGGA452.6855378E-720.9999987
CTTTTTT502.602792E-820.99999820
CTGTCTC554.2953165E-515.27272621
Produced by FastQC (version 0.11.2)