FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_38.3510000005fdd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_38.3510000005fdd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11250
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA620.5511111111111111No Hit
TGTTGCCGAACACCTGTCTCTTATACA190.1688888888888889No Hit
TGTTGCCGAACAGCTGTCTCTTATACA180.16No Hit
TGTTGCCGAACAGGGGGGGGTTTTTTT140.12444444444444445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCC9750.021.5779841
GTTGCCG9750.021.5779842
TGGTGCC403.6719066E-621.5779821
GGTGCCG403.6719066E-621.5779822
TTGCCGA9850.021.5779823
GTGCCGA403.6719066E-621.5779823
TTACCGA353.3163495E-521.577983
TGTTACC353.3163495E-521.577981
GTTACCG353.3163495E-521.577982
TGGTTTT608.203642E-1020.90666818
AACAAGG404.8537204E-620.9066689
TGCCGAA10850.020.9066684
TGTTTTT800.020.90666819
CATTTTT404.8537204E-620.90666819
TAGTTTT303.7205432E-420.90666818
GAGTTTT303.7205432E-420.90666818
CGAACAT2600.020.9066687
CGAACAA2250.020.9066687
GAACATA608.203642E-1020.9066688
GCGTTTT303.7205432E-420.90666818
Produced by FastQC (version 0.11.2)