FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_36.3510000005fd92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_36.3510000005fd92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12894
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA1270.9849542422832326No Hit
CAGAACGACAGTGGCTGTCTCTTATAC200.15511090429657207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG800.021.1411761
GAACGAC10950.021.1411763
AGAACGA10900.021.1411762
GCACGAC800.021.1411763
GACCGAC751.8189894E-1221.1411763
CAGACCG751.8189894E-1221.1411761
AGCACGA800.021.1411762
AGACCGA751.8189894E-1221.1411762
CAGAACG10900.021.1411761
CGACAGT12600.020.9766546
ACAGTGT1300.020.9766548
ACAGTGG2150.020.9766548
ACAGTGA557.185008E-920.9766548
TGGTTTT354.2010033E-520.97665418
ACAGTCG1450.020.9766548
ACAGTCC354.2010033E-520.9766548
ACAGTAT404.8042493E-620.9766548
ACAGTAG557.185008E-920.9766548
GGGTTTT659.276846E-1120.97665418
CAGTTTG354.2010033E-520.9766549
Produced by FastQC (version 0.11.2)