FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_35.3510000005fd78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_35.3510000005fd78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6841
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA861.257126151147493No Hit
CACAGAAGGACTGCTGTCTCTTATACA140.204648443210057No Hit
CACAGAAGGACTAGGGCTGTCTCTTAT80.116941967548604No Hit
CACAGAAGGACTCCTGTCTCTTATACA80.116941967548604No Hit
CACAGAAGGACTGGGGCTGTCTCTTAT70.1023242216050285No Hit
CACAGAAGGACTGGGGGGCGTTTTTTT70.1023242216050285No Hit
CACAGAAGGACTAAGGGCTGTCTCTTA70.1023242216050285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAAGG6150.021.2686583
ACAGAAG6000.021.2686562
CACCGAA250.00282934221.2686561
ACCGAAG250.00282934221.2686562
CACAGAA6000.021.2686561
CCGAAGG250.00282934221.2686563
AATTTTT303.4566142E-420.95588319
AGAAGGA6350.020.9558834
AGGACTG2850.020.9558837
AGGACTA1250.020.9558837
GGACTTT303.4566142E-420.9558838
GGACTTC353.8623213E-520.9558838
GGACTGG1150.020.9558838
GGACTCG303.4566142E-420.9558838
GGACTAG353.8623213E-520.9558838
GGACTAC303.4566142E-420.9558838
GGACTAA353.8623213E-520.9558838
CCGTTTT303.4566142E-420.95588318
GACTGGG353.8623213E-520.9558839
GACTGCT303.4566142E-420.9558839
Produced by FastQC (version 0.11.2)