FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_33.3510000005fd35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_33.3510000005fd35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCTCTGTCTCTTATACAC120.4559270516717325No Hit
AACGGAATGGCCTGTCTCTTATACACA110.4179331306990881No Hit
AACGGAATGGCTGCTGTCTCTTATACA90.34194528875379937No Hit
AACGGAATGGCTACTGTCTCTTATACA60.22796352583586624No Hit
AACGGAATGGCTCTGTCTCTTCTACAC60.22796352583586624No Hit
AACGGAATGGCTCCTGTCTCTTATACA60.22796352583586624No Hit
AACGGAATGGCTGTCTCTTATACACAT50.1899696048632219No Hit
AACGGAATGGCTTCTGTCTCTTATACA50.1899696048632219No Hit
AACGGAATGGCTCCTGTCTCTTATCCC40.1519756838905775No Hit
AACGGAATGGCTATTTATTATTTTTTT30.11398176291793312No Hit
AACGGAATGGCTGGGCGGGGTTTTTTT30.11398176291793312No Hit
AACGGAATGGCTCGGGCCTGTCTCTTA30.11398176291793312No Hit
AACGGAATGGCTGTCTCTTATCCACAT30.11398176291793312No Hit
AACGGAATGGCCTGTCTCTTCTACCCA30.11398176291793312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATG2350.021.7200013
AACGGAA2300.021.7200011
ACGGAAT2300.021.7200012
ATGGCTC552.7084752E-920.8846177
ATGGCTA502.822344E-820.8846177
GGTTTTT502.822344E-820.88461719
TGGCTTT250.002649486520.8846178
TGGCTGG250.002649486520.8846178
CTTTTTT502.822344E-820.88461720
ATGGCTT750.020.8846157
GAATGGC2550.020.8846155
AATGGCT2500.020.8846156
GGAATGG2500.020.8846154
ATGGCTG701.8189894E-1220.8846137
GTTTTTT1150.019.06856220
TTTTTTT2200.017.56206321
Produced by FastQC (version 0.11.2)