FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_32.3510000005fd28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_32.3510000005fd28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC250.6333924499619965No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC120.3040283759817583No Hit
GGATAGTTAGCCTGTCTCTTATACACA110.2786926779832784No Hit
GGATAGTTAGCCGGGGGGGCTTTTTTT60.15201418799087915No Hit
GGATAGTTAGCTGTCTCTTATACACAT60.15201418799087915No Hit
GGATAGTTAGCCGGCTGTCTCTTATAC50.12667848999239928No Hit
GGATAGTTAGCCGGGGGGGGTTTTTTT40.10134279199391943No Hit
GGATAGTTAGCCCCTGTCTCTTATACA40.10134279199391943No Hit
GGATAGTTAGCCGTAGGGGGTTTTTTT40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTT352.4877145E-521.722
GGATCGT352.4877145E-521.721
GGATAGT3000.021.721
ATAGTTA3000.021.723
ATCGTTA352.4877145E-521.723
GATAGTT3000.021.722
TAGCCGT303.166598E-420.8846178
TAGCCGG604.3473847E-1020.8846178
TAGCCGC303.166598E-420.8846178
TAGCCCT303.166598E-420.8846178
TAGCCCA303.166598E-420.8846178
TAGCCAG303.166598E-420.8846178
TAGCCAA303.166598E-420.8846178
CATTTTT303.166598E-420.88461719
TATTTTT303.166598E-420.88461719
GATTTTT303.166598E-420.88461719
GTTAGCC3900.020.8846176
TAGCCTT250.00291195320.8846158
TAGCCGA250.00291195320.8846158
TAGTTAG3450.020.8846154