FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_31.3510000005fd0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_31.3510000005fd0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15789
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAACCTGTCTCTTATACA210.1330039901197036No Hit
CAGACATCGCAAGCTGTCTCTTATACA170.10766989676356958No Hit
CAGACATCGCACTGTCTCTTATACACA160.10133637342453607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAT950.021.1730771
AGCCATC950.021.1730772
GAAATCG751.8189894E-1221.1730773
GACATCG13700.021.1730773
AGAAATC751.8189894E-1221.1730772
GCCATCG950.021.1730773
CAGACAT13600.021.1730771
CAGAAAT751.8189894E-1221.1730771
AGACATC13600.021.1730772
GGGTTTT751.8189894E-1220.9714318
CAATGGG250.003261601620.9714310
CAATGGA250.003261601620.9714310
CAGTTTT250.003261601620.9714318
GCAAGGT506.54054E-820.971439
GCAAGCC250.003261601620.971439
GCAAGAT250.003261601620.971439
AAATCGC751.8189894E-1220.971434
CAACTGG250.003261601620.9714310
GGATTTT506.54054E-820.9714318
CATTTTT1000.020.9714319
Produced by FastQC (version 0.11.2)