FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_3.3510000005f989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_3.3510000005f989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC341.3250194855806703No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC250.9742790335151987No Hit
GAGGAACAGAGCTGTCTCTTATACACA110.4286827747466875No Hit
GAGGAACAGAGTAGCTGTCTCTTATAC60.23382696804364772No Hit
GAGGAACAGAGTGGCTGTCTCTTATAC40.1558846453624318No Hit
GAGGAACAGAGTGTGGGGGGTTTTTTT30.11691348402182386No Hit
GAGGAACAGAGTGGCGGCTGTCTCTTA30.11691348402182386No Hit
GAGGAACAGAGTTGAGGCTGTCTCTTA30.11691348402182386No Hit
GAGGAACAGAGTGAGGGCTGTCTCTTA30.11691348402182386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCAC250.002297525321.361
GGCACAG250.002297525321.363
AGGCACA250.002297525321.362
GGAACAG2200.021.363
GAGGAAC2150.021.3599991
AGGAACA2150.021.3599992
TTTTTTT1200.020.94117721
GTTTTTT502.681736E-820.94117720
CAGAGTT402.7866572E-620.9411777
CAGAGTG1000.020.9411777
CAGAGTA402.7866572E-620.9411777
AGAGTGG452.759498E-720.9411778
AGAGTGA250.002593452320.9411778
AGAGTCG302.6982988E-420.9411778
AGAGTCC250.002593452320.9411778
ATTTTTT250.002593452320.94117720
AGTTTTT250.002593452320.94117719
GCACAGA250.002593452320.9411774
CACAGAG250.002593452320.9411775
CTTTTTT452.759498E-720.94117720
Produced by FastQC (version 0.11.2)