FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_28.3510000005fc93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_28.3510000005fc93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11249
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC1141.0134234154147037No Hit
AGAGGACGAGCTGTCTCTTATACACAT680.6044981776157881No Hit
AGAGGACGAGATGCTGTCTCTTATACA270.2400213352297982No Hit
AGAGGACGAGATCTGTCTCTTCTACAC260.2311316561472131No Hit
AGAGGACGAGCTGTCTCTTATCCACAT140.12445550715619166No Hit
AGAGGACGAGATCCTGTCTCTTATACA130.11556582807360655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGACG9900.021.4954132
AGAGGAC9900.021.4954131
CGGACGA707.2759576E-1221.4954133
GCGGACG707.2759576E-1221.4954132
AGCGGAC707.2759576E-1221.4954131
AGGACGA9950.021.495413
GATGGGG404.8244347E-620.91964310
TTGTTTT354.2259988E-520.91964318
GTGTTTT303.7035294E-420.91964318
TGATTTT303.7035294E-420.91964318
TTCTGTC303.7035294E-420.91964321
GGTTTTT1150.020.91964319
GAGTTTT404.8244347E-620.91964318
ACTTTTT354.2259988E-520.91964319
TGCTTTT354.2259988E-520.91964318
ACGAGAT10950.020.9196436
CCTTTTT404.8244347E-620.91964319
AGATGTT303.7035294E-420.9196439
GCGTTTT354.2259988E-520.91964318
AGATGCC404.8244347E-620.9196439
Produced by FastQC (version 0.11.2)