FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_24.3510000005fc1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_24.3510000005fc1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8290
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGCTGTCTCTTATACACA961.158021712907117No Hit
GTGTAGTGTGGAGGCTGTCTCTTATAC861.037394451145959No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC410.4945717732207479No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC400.48250904704463204No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC370.4463208685162847No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC330.3980699638118214No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA290.3498190591073582No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC250.30156815440289503No Hit
GTGTAGTGTGGACCTGTCTCTTATACA240.28950542822677927No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC210.25331724969843183No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC170.20506634499396864No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC150.18094089264173704No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC140.16887816646562123No Hit
GTGTAGTGTGGATCTGTCTCTTATACA130.15681544028950542No Hit
GTGTAGTGTGGACTCTGTCTCTTATAC130.15681544028950542No Hit
GTGTAGTGTGGATACTGTCTCTTATAC120.14475271411338964No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC100.12062726176115801No Hit
GTGTAGTGTGGAAACTGTCTCTTATAC90.10856453558504221No Hit
GTGTAGTGTGGCTGTCTCTTCTACCCA90.10856453558504221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGT504.6660716E-821.3333341
GTCGTGT504.6660716E-821.3333343
GTGTAGT7250.021.3333341
TGTAGTG7250.021.3333342
TGTCGTG504.6660716E-821.3333342
GTAGTGT7550.021.3333343
AATTTTT250.003154528620.94545619
ACGTTTT250.003154528620.94545618
TTTTTTT5650.020.94545621
GTTTTTT3100.020.94545620
GGTTTTT1300.020.94545619
TCGTGTG505.7012585E-820.9454564
TGGAGTC250.003154528620.9454569
TGGAGGT250.003154528620.9454569
TGGAGCA250.003154528620.9454569
TGGAGAT250.003154528620.9454569
CGGGTTT250.003154528620.94545617
CGTGTGG505.7012585E-820.9454565
TGTGGCT250.003154528620.9454567
TGTGGAA1450.020.9454567
Produced by FastQC (version 0.11.2)