FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_18.3510000005fb51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_18.3510000005fb51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14275
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA1150.8056042031523643No Hit
GCATTGGATACCCTGTCTCTTATACAC610.42732049036777586No Hit
GCATTGGATACCGGCTGTCTCTTATAC610.42732049036777586No Hit
GCATTGGATACCGCCTGTCTCTTATAC420.29422066549912435No Hit
GCATTGGATACCTGCTGTCTCTTATAC390.2732049036777583No Hit
GCATTGGATACCGTCTGTCTCTTATAC380.266199649737303No Hit
GCATTGGATACCAGCTGTCTCTTATAC320.22416812609457093No Hit
GCATTGGATACCCCTGTCTCTTATACA300.21015761821366027No Hit
GCATTGGATACCCCCTGTCTCTTATAC270.18914185639229422No Hit
GCATTGGATACCCGCTGTCTCTTATAC250.17513134851138354No Hit
GCATTGGATACCACCTGTCTCTTATAC220.15411558669001751No Hit
GCATTGGATACCCTGTCTCTTCTACAC210.14711033274956217No Hit
GCATTGGATACCATCTGTCTCTTATAC200.14010507880910683No Hit
GCATTGGATACCTCCTGTCTCTTATAC200.14010507880910683No Hit
GCATTGGATACTGTCTCTTATACACAT190.1330998248686515No Hit
GCATTGGATACCGCTGTCTCTTATACA190.1330998248686515No Hit
GCATTGGATACCGACTGTCTCTTATAC190.1330998248686515No Hit
GCATTGGATACCTTCTGTCTCTTATAC190.1330998248686515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGA12450.021.4532382
TTTGGAT403.993213E-621.4532383
GCATTGG12450.021.4532381
GCTTTGG403.993213E-621.4532381
GCCTTGG750.021.4532361
ATTGGAT12500.021.4532363
CCTTGGA750.021.4532362
CTTGGAT750.021.4532363
ACCGGGG557.565177E-920.92631710
GATACCG5400.020.9263177
CGCTTTT303.7656003E-420.92631718
GTTTTTT4800.020.92631720
GAGTTTT303.7656003E-420.92631718
TACCGGT303.7656003E-420.9263179
TACCGCC303.7656003E-420.9263179
GGCTTTT303.7656003E-420.92631718
ACCCTGT303.7656003E-420.92631710
GCGTTTT303.7656003E-420.92631718
ATGTTTT303.7656003E-420.92631718
AGGTTTT303.7656003E-420.92631718
Produced by FastQC (version 0.11.2)