FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_17.3510000005fb37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_17.3510000005fb37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC820.7162197571840335No Hit
AACGTGCGTATGGGCTGTCTCTTATAC440.38431304044021314No Hit
AACGTGCGTATGCTGTCTCTTCTACAC430.375578653157481No Hit
AACGTGCGTATCTGTCTCTTATACACA380.33190671674382044No Hit
AACGTGCGTATGGCTGTCTCTTATACA360.3144379421783562No Hit
AACGTGCGTATGCCTGTCTCTTATACA310.2707660057646956No Hit
AACGTGCGTATGTCTGTCTCTTATACA200.17468774565464232No Hit
AACGTGCGTATGTGCTGTCTCTTATAC190.16595335837191022No Hit
AACGTGCGTATGGGGCTGTCTCTTATA160.13975019652371387No Hit
AACGTGCGTATGGCCTGTCTCTTATAC140.12228142195824963No Hit
AACGTGCGTATGACCTGTCTCTTATAC140.12228142195824963No Hit
AACGTGCGTATGAGCTGTCTCTTATAC120.1048126473927854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC10450.021.4864861
CGTGCGT10600.021.4864863
AATGTGC250.00278083921.4864861
TGTGCGT250.00278083921.4864863
ACGTGCG10450.021.4864862
TTGTTTT250.003251768620.92105318
GTATGTT404.8344336E-620.9210538
GTATGTG950.020.9210538
GTATGTC404.8344336E-620.9210538
GTATGTA506.301161E-820.9210538
ATGGGGT404.8344336E-620.92105310
ATGGGCA250.003251768620.92105310
GTATGGT751.8189894E-1220.9210538
GTATGGC900.020.9210538
GTATGGA800.020.9210538
ATGGGAG250.003251768620.92105310
GTATGCT455.5212513E-720.9210538
GTATGCG800.020.9210538
GTATGCC250.003251768620.9210538
GTATGCA404.8344336E-620.9210538
Produced by FastQC (version 0.11.2)