FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_11.3510000005fa88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_11.3510000005fa88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA90.2280212819863187No Hit
AAGTGCCTCAGTGCTGTCTCTTATACA80.20268558398783887No Hit
AAGTGCCTCAGTAGCTGTCTCTTATAC40.10134279199391943No Hit
AAGTGCCTCAGTCTGTCTCTTATACAC40.10134279199391943No Hit
AAGTGCCTCAGTGGCGGGTGTTTTTTT40.10134279199391943No Hit
AAGTGCCTCAGTATCTGTCTCTTATAC40.10134279199391943No Hit
AAGTGCCTCAGTACCTGTCTCTTATAC40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCC3550.021.7200011
GTGCCTC3600.021.723
AGTGCCT3600.021.4183312
TCAGTTT303.166598E-420.8846178
TCAGTAT303.166598E-420.8846178
CTCAGTT1100.020.8846177
CCTCAGT3900.020.8846176
ATTTTTT604.3473847E-1020.88461720
GCCTCAG3750.020.8846155
TCAGTTG250.00291195320.8846158
TCAGTGA403.6588535E-620.8846158
TGCCTCA3750.020.8846154
TCAGTAC250.00291195320.8846158
CTCAGTA800.020.8846157
CAGTGGA250.00291195320.8846159
CTTTTTT403.6588535E-620.88461520
GTCTGTC353.418015E-520.88461320
TCAGTTA353.418015E-520.8846138
TCAGTGT353.418015E-520.8846138
TCAGTGG453.8809412E-720.8846138
Produced by FastQC (version 0.11.2)