FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate7_10.3510000005fa6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate7_10.3510000005fa6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC221.7614091273018415No Hit
ACGTCTCTGCCTGTCTCTTATACACAT90.7205764611689351No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC80.6405124099279423No Hit
ACGTATCTGCATCTGTCTCTTCTACAC40.32025620496397117No Hit
ACGTCTCTGCATAGGGGATGTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGGTAGGGGTCTGTCT20.16012810248198558No Hit
ACGTCTCTGCATGGGGGCGGTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATTGGGACCATTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATCTCGTCGGTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGCTGTCTCTTATACA20.16012810248198558No Hit
ACGTCTCTGCATCAGGGCCTGTCTCTT20.16012810248198558No Hit
ACGTCTCTGCATAGGGCTGTCTCTTAT20.16012810248198558No Hit
ACGTCTCTGCATGAGCCCGGTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGAGGTTGGTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGCGGGGCCTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGGGCGGGGTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGCCTATGATTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGGCTATCTGTCTCTT20.16012810248198558No Hit
ACGTCTCTGCATGGAGTAGCTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGCCCTGTCTCTTATA20.16012810248198558No Hit
ACGTCTCTGCATAGGGGAGCTTTTTTT20.16012810248198558No Hit
ACGTCTCTGCATGGGGCGGGTTTTTTT20.16012810248198558No Hit
ATGTCTCTGCATCTGTCTCTTATACAC20.16012810248198558No Hit
ACGTCTCTGCATGGGGGGCTGTCTCTT20.16012810248198558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGCA1050.021.05
ACGTCTC1050.021.01
TCTGCAT1200.021.06
GTCTCTG1050.021.03
TCTCTGC1050.021.04
CTGCATC301.6086693E-421.07
CGTCTCT1050.021.02
CTTTTTT351.3680683E-521.020
TTTTTTT1000.020.99999821
GTTTTTT506.044502E-920.99999820
CTGCATT250.001803158120.9999987
CTGCATG506.044502E-920.9999987
GCTTTTT250.001803158120.99999819
TGCATGG300.005220918517.58
Produced by FastQC (version 0.11.2)