FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_96.3510000005f92c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_96.3510000005f92c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15592
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC1831.1736788096459723No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC630.40405336069779374No Hit
TAAGCGTGGAACTGTCTCTTATACACA190.12185736275012826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT13650.021.4210531
TAAGAGT250.0028792321.4210531
TACGCGT850.021.4210511
AAGAGTG250.002935082421.350822
AGAGTGG250.002935082421.350823
AAGCGTG13700.021.350822
AGCGTGG13850.021.350823
TGGTTTT354.335789E-520.93890818
AACGCGG250.00328921620.93890810
GTGTTTT455.753991E-720.93890818
GCCTTTT250.00328921620.93890818
AGCTTTT250.00328921620.93890818
AATTTTT1000.020.93890819
GAGTGGA250.00328921620.9389084
GGCGTTT250.00328921620.93890817
TTTCTGT354.335789E-520.93890821
TGATTTT354.335789E-520.93890818
GAACGTT354.335789E-520.9389089
GAACGTG455.753991E-720.9389089
GAACGTC250.00328921620.9389089
Produced by FastQC (version 0.11.2)