FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_95.3510000005f902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_95.3510000005f902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38425
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC510.13272608978529604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAGA505.8529622E-821.3829781
TAGCCGA2750.021.3829781
TAGCAGA33500.021.3829781
AGCAGAC33500.021.3829782
AGCCGAC2750.021.3829782
TGCAGAC505.8529622E-821.3829782
GCCGACT2750.021.3829783
ATCAGAC404.4331664E-621.3829772
TCAGACT404.4331664E-621.3829773
TATCAGA404.4331664E-621.3829771
GCAGACT34000.021.3515343
TTATTTT558.611096E-920.93750218
TGATTTT558.611096E-920.93750218
CAATTTT558.611096E-920.93750218
TAATTTT558.611096E-920.93750218
CTGGAGA2100.020.9375028
CTGGACC1050.020.9375028
CTGGAAT1750.020.9375028
TGGATTA558.611096E-920.9375029
TGGACGA558.611096E-920.9375029
Produced by FastQC (version 0.11.2)