FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_94.3510000005f8e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_94.3510000005f8e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70334
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA2350.3341200557340689No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1050.1492876844769244No Hit
TACTGCAAGGAGCTGTCTCTTATACAC710.10094691045582506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAAG751.8189894E-1221.4729943
TTCTGCA950.021.4729941
TATTGCA751.8189894E-1221.4729941
TCTGCAA950.021.4729942
ATTGCAA751.8189894E-1221.4729942
CTGCAAG65400.021.4729943
CTACAAG1050.021.4729923
ACTGCAA64500.021.4729922
CTCCAAG505.7716534E-821.4729923
ACTCCAA505.7716534E-821.4729922
TAATGCA505.7716534E-821.4729921
TACTGCA64500.021.4729921
AATGCAA505.7716534E-821.4729922
TACTCCA505.7716534E-821.4729921
TACTACA1050.021.4729921
ACTACAA1050.021.4729922
TTGTTTT1100.020.92318718
TGGTTTT1100.020.92318718
GGAGAGA558.996722E-920.9231879
GGAGACG558.996722E-920.9231879
Produced by FastQC (version 0.11.2)