FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_87.3510000005f803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_87.3510000005f803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24345
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA430.17662764428013966No Hit
ACTTCGTCAGCTGTCTCTTATACACAT410.16841240501129595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT404.4638455E-621.3033491
ACATCGT404.4638455E-621.3033491
ACCTCGT404.4638455E-621.3033491
ACTTCGT20250.021.3033471
ACTTAGT2050.021.3033471
TTCGTCA20450.021.2588733
GTCGTCA404.5461184E-621.2588733
CCTCGTC404.5461184E-621.2588732
CTTAGTC2050.021.2588732
CGTCGTC404.5461184E-621.2588732
ATCGTCA404.5461184E-621.2588733
TTAGTCA2050.021.2588733
CTCGTCA404.5461184E-621.2588733
CATCGTC404.5461184E-621.2588732
CTTCGTC20300.021.2588712
CGTCAGC22000.020.9526775
TCGTCAG22000.020.9526774
CTTTTGG250.003330035820.95267511
CAGCTCT800.020.9526758
CAGCTCG1750.020.9526758
Produced by FastQC (version 0.11.2)