FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_86.3510000005f7e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_86.3510000005f7e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21644
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA610.2818333025318795No Hit
TGTTGCCGAACAGGGGGGGGTTTTTTT260.12012566993162077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCCG303.1969856E-421.5095252
TGTTCCC303.1969856E-421.5095251
TTCCCGA303.1969856E-421.5095253
TGGTGCC657.6397555E-1121.5095251
GGTGCCG657.6397555E-1121.5095252
GTGCCGA657.6397555E-1121.5095253
TGTTGCC19250.021.5095231
TTACCGA404.066629E-621.5095233
TGTTACC404.066629E-621.5095231
GTTACCG404.066629E-621.5095232
GTTGCCG19250.021.5095232
TTGCCGA19350.021.5095233
GTTCTGT303.811802E-420.96055820
TTCTGTC405.098629E-620.96055621
AACAATT303.872342E-420.9120399
AACAATG303.872342E-420.9120399
AACAAGG609.567884E-1020.9120399
AACAAGC354.48294E-520.9120399
ACATGGT303.872342E-420.91203910
TTATTTT303.872342E-420.91203918
Produced by FastQC (version 0.11.2)