FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_85.3510000005f7ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_85.3510000005f7ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42768
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA2350.5494762439206884No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC600.14029180695847362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA37100.021.3214282
CTTTAGT608.1490725E-1021.3214281
GCTAGTA701.0913936E-1121.3214282
CTCTCGT2700.021.3214281
CTCTAGT37150.021.3214281
TCTCGTA2700.021.3214282
TTTAGTA608.1490725E-1021.3214282
CTAGTAC38100.021.3214283
TTAGTAC608.1490725E-1021.3214283
CTCGTAC2700.021.3214283
TGGTTTT1300.020.9473718
ACCGATA1850.020.947378
ACCGAGT4300.020.947378
ACCGAGC2750.020.947378
ACCGACC1850.020.947378
TGCTTTT651.200533E-1020.9473718
CCGAGGA651.200533E-1020.947379
CCGAGCC651.200533E-1020.947379
CCGAGAG651.200533E-1020.947379
CCGAGAA651.200533E-1020.947379
Produced by FastQC (version 0.11.2)